Core facility extracellular vesicles

Our lab is fully equipped with a state-of-the-art facility for the isolation, purification and downstream characterisation of extracellular vesicles.

The core facility is accessible to members of FAU – please get in touch with us should you want to have support.

Figure prepared with BioRender (academic license FAU)
Available instrumentation:

  • Ultracentrifuge Optima XPN80 (Beckman) with rotors 32 SW and 45 Ti for EV isolation
  • Äkta Start and Äkta Pure for EV purification
  • Nanoparticle Tracking Analysis Zetaview Twin (Particle Metrix) for EV characterisation
  • Cytoflex SRT (Beckman) for analysis and sorting of S2 bacteria
  • Confocal laser scanning microscope (Evident) with FLIM/FCS module (PicoQuant) for cell and bacterial analysis
  • Automated live cell imager FX (Lionheart) for high-content cell imaging

 

(Our large-scale research equipments FACS and CLSM are funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – project number 511550821)