Jennifer Munkert
Dr. Jennifer Munkert
Education and training
2004 – 2009 | Studies of biological Sciences at the Friedrich-Alexander-Universität Erlangen-Nürnberg, Diploma-Thesis Supervisor: Prof. Dr. W. Kreis |
2010 – 2014 | PhD Student in the Group of Prof. Dr. W. Kreis, Chair of Pharmaceutical Biology, at the Friedrich-Alexander-Universität Erlangen-Nürnberg. PhD Supervisor: Prof. Dr. W. Kreis |
Feb/March 2012 | Visiting scientist at the Flandern Institute of Biotechnology (VIB), Ghent, Belgium, in Professor Alain Goossens‘ group |
March 2013 | Visiting scientist at Institute of Biology at Leiden University, Sylvius Laboratory (IBL, Leiden, Netherlends), in Professor Johan Memenlink’s group |
July – Oct 2013 | Visiting scientist at the Faculty of Pharmacy at the University Fedral of Minas Gerais, Belo Horizonte Brasil, in Professor F.C. Braga’s group |
Professional positions
Aug – Nov 2014/2015 | Visiting scientist at the Faculty of Pharmacy at the University Fedral of Minas Gerais, Belo Horizonte Brasil, in Professor F.C. Braga’s group |
2015 | PostDoc in the Group of Prof. Dr. Rainer Buchholz, Lehrstuhl für Bioverfahrenstechnik, at the Friedrich-Alexander-Universität Erlangen-Nürnberg |
2016 | PostDoc in the Group of Prof. Dr. Wolfgang Kreis, Lehrstuhl für Pharmazeutische Biologie, at the Friedrich-Alexander-Universität Erlangen-Nürnberg |
Publications
2022
Effects of Lipophilicity and Structural Features on the Antiherpes Activity of Digitalis Cardenolides and Derivatives
In: Chemistry & Biodiversity (2022)
ISSN: 1612-1872
DOI: 10.1002/cbdv.202200411
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Knockout of arabidopsis thaliana vep1, encoding a prise (Progesterone 5β-reductase/iridoid synthase-like enzyme), leads to metabolic changes in response to exogenous methyl vinyl ketone (mvk)
In: Metabolites 12 (2022), Art.Nr.: 11
ISSN: 2218-1989
DOI: 10.3390/metabo12010011
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2021
Identifying structural features for changing substrate preferences in two Plantago PRISEs (pro-gesterone 5 beta-reductase and / or iridoid synthase)
In: PLANTA MEDICA, STUTTGART: 2021
DOI: 10.1055/s-0041-1736952
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Restoring cardenolide accumulation in RNAi-mediated DlP5 beta R1 and DlP5 beta R2 knockdown shoot culture lines of Digitalis lanata and studying the effects of precursor feeding on cardenolide free dark-cultured D. lanata shoots
In: PLANTA MEDICA, STUTTGART: 2021
DOI: 10.1055/s-0041-1736902
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RNAi-mediated gene knockdown of progesterone 5β-reductases in Digitalis lanata reduces 5β-cardenolide content
In: Plant Cell Reports (2021)
ISSN: 0721-7714
DOI: 10.1007/s00299-021-02707-3
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Overexpression of 3 beta-hydroxysteroid dehydrogenases in Digitalis lanata shoot cultures
In: PLANTA MEDICA, STUTTGART: 2021
DOI: 10.1055/s-0041-1736957
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21-Hydroxypregnane 21-O-malonylation, a crucial step in cardenolide biosynthesis, can be achieved by substrate-promiscuous BAHD-type phenolic glucoside malonyltransferases from Arabidopsis thaliana and homolog proteins from Digitalis lanata
In: Phytochemistry 187 (2021), Art.Nr.: 112710
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2021.112710
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Cytotoxicity of glucoevatromonoside alone and in combination with chemotherapy drugs and their effects on Na+,K+-ATPase and ion channels on lung cancer cells
In: Molecular and Cellular Biochemistry (2021)
ISSN: 0300-8177
DOI: 10.1007/s11010-020-04040-x
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2020
Investigation of the cytotoxic activity of two novel digitoxigenin analogues on H460 lung cancer cells
In: Anti-Cancer Drugs 31 (2020), S. 452-462
ISSN: 0959-4973
DOI: 10.1097/CAD.0000000000000872
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Elucidation of the mechanism of anti-herpes action of two novel semisynthetic cardenolide derivatives.
In: Archives of Virology (2020), S. 1385-1396
ISSN: 0304-8608
DOI: 10.1007/s00705-020-04562-1
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Semisynthetic Cardenolides Acting as Antiviral Inhibitors of Influenza A Virus Replication by Preventing Polymerase Complex Formation
In: Molecules (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25204853
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Digitoxigenin presents an effective and selective antileishmanial action against Leishmania infantum and is a potential therapeutic agent for visceral leishmaniasis
In: Parasitology Research (2020)
ISSN: 0932-0113
DOI: 10.1007/s00436-020-06971-2
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Cardenolide and glucosinolate accumulation in shoot cultures of Erysimum crepidifolium Rchb.
In: In Vitro Cellular & Developmental Biology-Plant (2020)
ISSN: 1054-5476
DOI: 10.1007/s11627-020-10135-3
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2019
Potential anti-herpes and cytotoxic action of novel semisynthetic digitoxigenin-derivatives
In: European Journal of Medicinal Chemistry 167 (2019), S. 546-561
ISSN: 0223-5234
DOI: 10.1016/j.ejmech.2019.01.076
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Potential anti-herpes and cytotoxic action of novel semisynthetic digitoxigenin-derivatives
67th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) (Innsbruck, 1. September 2019 - 5. September 2019)
In: PLANTA MEDICA, STUTTGART: 2019
DOI: 10.1055/s-0039-3399709
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Exploiting enzyme promiscuity to shape plant specialized metabolism
In: Journal of Experimental Botany 70 (2019), S. 1435-1445
ISSN: 0022-0957
DOI: 10.1093/jxb/erz025
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RNAi mediated knock down of 3 beta-hydroxysteroid dehydrogenases in Digitalis lanata shoot cultures
67th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) (Innsbruck, 1. September 2019 - 5. September 2019)
In: PLANTA MEDICA, STUTTGART: 2019
DOI: 10.1055/s-0039-3399800
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New 99mTc-Labeled Digitoxigenin Derivative for Cancer Cell Identification
In: ACS Omega (2019)
ISSN: 2470-1343
DOI: 10.1021/acsomega.9b03167
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Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae
In: MicrobiologyOpen (2019), Art.Nr.: e925
ISSN: 2045-8827
DOI: 10.1002/mbo3.925
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BAHD-like malonyltransferase genes from Digitalis lanata and Arabidopsis thaliana and their putative role in cardenolide biosynthesis
67th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research (GA) (Innsbruck, 1. September 2019 - 5. September 2019)
In: PLANTA MEDICA, STUTTGART: 2019
DOI: 10.1055/s-0039-3399788
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2018
Long-circulating and fusogenic liposomes loaded with a glucoevatromomoside derivative induce potent antitumor response
In: Biomedicine & Pharmacotherapy (2018), S. 1152 - 1161
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2018.09.109
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PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism.
In: Phytochemistry 156 (2018), S. 9-19
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2018.08.012
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Cardiac Glycoside Glucoevatromonoside Induces Cancer Type-specific Cell Death
In: Frontiers in Pharmacology 9 (2018), Art.Nr.: 70
ISSN: 1663-9812
DOI: 10.3389/fphar.2018.00070
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Cytotoxicity of AMANTADIG - a semisynthetic digitoxigenin derivative - alone and in combination with docetaxel in human hormone-refractory prostate cancer cells and its effect on Na+/K+ -ATPase inhibition.
In: Biomedicine & Pharmacotherapy 107 (2018), S. 464-474
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2018.08.028
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2017
Biossíntese de metabólitos primários e secundários
In: Letícia Bispo de Lima (Hrsg.): Farmacognosia do Produto Natural ao Medicamento, Porto Alegre, Brazil: Artmed Editora Ltda., 2017, S. 147 - 166
ISBN: 978-85-8271-359-4
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Herzglykoside und ihr Potenzial für die Tumortherapie
In: Deutsche Zeitschrift für Onkologie 49 (2017), S. 129-135
ISSN: 0931-0037
DOI: 10.1055/s-0043-115681
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Production of the Cytotoxic Cardenolide Glucoevatromonoside by Semisynthesis and Biotransformation of Evatromonoside by a Digitalis lanata Cell Culture.
In: Planta medica (2017)
ISSN: 1439-0221
DOI: 10.1055/s-0043-109557
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A new semisynthetic cardenolide analog 3β-[2-(1-amantadine)-1-on-ethylamine]-digitoxigenin (AMANTADIG) affects G2/M cell cycle arrest and miRNA expression profiles and enhances proapoptotic survivin-2B expression in renal cell carcinoma cell lines
In: Oncotarget 8 (2017), S. 11676-11691
ISSN: 1949-2553
DOI: 10.18632/oncotarget.14644
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Cytotoxic and cytostatic effects of digitoxigenin monodigitoxoside (DGX) in human lung cancer cells and its link to Na,K-ATPase
In: Biomedicine & Pharmacotherapy 97 (2017), S. 684-696
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2017.10.128
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2016
The catalytic mechanism of the 3-ketosteroid isomerase of Digitalis lanata involves an intramolecular proton transfer and the activity is not associated with the 3 beta-hydroxysteroid dehydrogenase activity
In: Tetrahedron Letters 57 (2016), S. 1567-1571
ISSN: 0040-4039
DOI: 10.1016/j.tetlet.2016.02.099
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2015
Steroid 5β-reductase from leaves of Vitis vinifera: molecular cloning, expression and modelling
In: Journal of Agricultural and Food Chemistry 63 (2015), S. 10112–10120
ISSN: 0021-8561
DOI: 10.1021/acs.jafc.5b04261
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Progesterone 5β-reductase genes of the Brassicaceae family as function-associated molecular markers
In: Plant Biology 17 (2015), S. 1113-1122
ISSN: 1435-8603
DOI: 10.1111/plb.12361
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Iridoid Synthase Activity is Common among the Plant Progesterone 5β-Reductase Family
In: Molecular Plant 8 (2015), S. 136-152
ISSN: 1674-2052
DOI: 10.1016/j.molp.2014.11.005
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Progesterone 5β-reductases/iridoid synthases (PRISE): gatekeeper role of highly conserved phenylalanines in substrate preference and trapping is supported by molecular dynamics simulations
In: Journal of Biomolecular Structure & Dynamics (2015), S. 1-14
ISSN: 0739-1102
DOI: 10.1080/07391102.2015.1088797
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2014
Identification and stress-induced expression of three 3β-hydroxysteroid dehydrogenases from Erysimum crepidifolium Rchb. and their putative role in cardenolide biosynthesis
In: Phytochemistry 100 (2014), S. 26-33
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2014.01.006
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2011
Progesterone 5β-reductase of Erysimum crepidifolium: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant protein
In: Phytochemistry 72 (2011), S. 1710-1717
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2011.06.007
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2010
Highly conserved progesterone 5β-reductase (P5βR) genes, from 5β-cardenolide-free and 5β-cardenolide-producing angiosperms.
In: Phytochemistry 71 (2010), S. 1495-1505
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2010.06.004
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